This manual describes the use of rat adipocyte differentiation protein (ADP) enzyme-linked immunosorbent assay kit, which is used for quantitative measurement of ADP content in rat serum, plasma, tissue homogenates, cell culture supernatants or other relevant fluids.
The kit uses a sandwich ELISA principle: a purified capture antibody is coated on a 96‑well plate, followed by addition of the ADP antigen, a biotin‑labeled detection antibody, HRP‑conjugated streptavidin and TMB substrate, producing a yellow color proportional to ADP concentration.
The document details reagent preparation, sample handling, assay procedure, plate washing, calculation of concentrations, and important precautions such as proper washing, avoiding hemolysis, and storage conditions for reagents and samples. This manual is written in Chinese.
| Plate type | 96‑well |
|---|---|
| Detection range | 3.12 ng/mL – 200 ng/mL |