Key facts
- Manuals
- 1
- Languages
- ZH
- Last added
- 2012-01-12
Featured XbaI内切酶 manuals
XbaI Endonuclease User Manual
The document describes the XbaI restriction enzyme, its activity (1500 U per vial) and recommended reaction conditions. It includes detailed buffer formulations (10 mM Tris‑HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 10 mM 2‑mercaptoethanol, 0.2 mg/ml BSA, glycerol) and a 1× working buffer (33 mM Tris‑acetate pH 7.9, 10 mM Mg‑acetate, 66 mM K‑acetate, 0.1 mg/ml BSA).
Instructions cover enzyme activation, DNA amount limits, incubation temperature (37 °C for 1 h), and storage (‑20 °C). Safety notes and troubleshooting tips are also provided. This manual is written in Chinese.
Covers: Enzyme Overview · Buffer Preparation · Reaction Setup · Incubation Conditions · Storage and Stability · Safety Precautions
Latest XbaI内切酶 manuals
XbaI Endonuclease User Manual
User Manual 1 pages · 123 KB · ZHThis manual provides usage instructions, buffer recipes, and storage guidelines for the XbaI restriction enzyme (1500 U).
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in XbaI内切酶 manuals
What temperature should XbaI be incubated at for digestion?
Incubate the reaction at 37 °C for about 1 hour.
What is the composition of the 10× Buffer Y?
The 10× Buffer Y contains 33 mM Tris‑acetate (pH 7.9 at 37 °C), 10 mM magnesium acetate, 66 mM potassium acetate, and 0.1 mg/ml BSA.