NEWPEP Manuals

NEWPEP FastClone Kit Manual 70311-01/70311-02

The FastClone Kit uses homologous recombination to insert PCR products into any position of a vector without restriction enzymes or T4 DNA ligase. It enables cloning of DNA fragments ranging from 100 bp to 20 kb, achieving positive rates of over 90 % and reducing reaction time to about 30 minutes.

The kit includes FastClone Enzyme, 10X FastClone Buffer, EcoRI‑linearized pUC19 plasmid, and a 1 kb positive‑control PCR product. Detailed protocols cover vector linearization, preparation of insert fragments with 15 bp vector‑matching overhangs, and directional subcloning with a recommended 1:5 vector‑to‑insert molar ratio. This manual is written in Chinese.

Covers: Product Overview · Key Features · Kit Components · Applications · Example Procedure · Directional Subcloning Protocol