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- Manuals
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- EN
- Last added
- 2026-10-01
Featured Biosearch Technologies manuals
Biosearch Technologies CAL FLUOR and Quasar Dyes Thermal Cycler Spectral Calibration Instructions
This manual explains why spectral calibration is required for real‑time qPCR instruments and how it enables accurate multiplexed assays by distinguishing overlapping fluorescent signals. It details the preparation, storage, and protection of calibration standards, as well as optional steps such as reaction‑plate centrifugation.
The document provides instrument‑specific calibration procedures for a range of platforms (ABI Prism 7700, ABI 7500, Bio‑Rad iCycler IQ/iQ5/Opticon, Cepheid SmartCycler II, Eppendorf Mastercycler ep Realplex, Roche LightCycler 480) and includes tables of recommended dye concentrations, catalogue numbers, and handling guidelines.
Covers: Why is spectral calibration necessary? · Instrument‑specific calibration instructions · Normalised emission spectra of calibration dyes · Calibration standards – tube contents · Calibration concentrations · Preparation of calibration standards for use
Latest Biosearch Technologies manuals
Biosearch Technologies CAL FLUOR and Quasar Dyes Thermal Cycler Spectral Calibration Instructions
User Manual 24 pages · 517 KB · ENStep‑by‑step guide for calibrating thermal cyclers to recognize CAL FLUOR and Quasar dye spectra, with instrument‑specific procedures and handling instructions.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in Biosearch Technologies manuals
Why is spectral calibration necessary for real‑time qPCR instruments?
Most real‑time qPCR instruments require dye calibration to distinguish fluorescent signals, especially for multiplexed assays where overlapping signals must be resolved.
From: Biosearch Technologies CAL FLUOR and Quasar Dyes Thermal Cycler Spectral Calibration Instructions
How should calibration standards be stored?
Aliquot each standard into individual microvials and store frozen at -20 °C or -80 °C, minimizing freeze‑thaw cycles.
From: Biosearch Technologies CAL FLUOR and Quasar Dyes Thermal Cycler Spectral Calibration Instructions