The document describes the two-step chromatography process for the purification of monoclonal antibodies (MAb) using the A..KTATMavant25 system controlled by ImaginationatWork UNICORNTM6 software. The system utilizes MabSelectSuReTM, a protein A-based chromatography column, for the initial capture step, and CaptoTMadhere, a multimodal anion exchange resin, for the second polishing step to reduce impurities. The software-integrated experimental design (DoE) in UNICORNTM6 simplifies the optimization of process conditions, resulting in high yield and purity of the target MAb.