The present invention relates to a PCR detection kit for African swine fever virus, characterized in that the kit comprises: primers, Ex Taq buffer, Ex Taq Hot-start DNA polymerase, MgCl2, dNTP, wherein the primer sequence is: Prime r:F 5'- TAGAGGACGATACGATGGT-3', R 5'-CGAATGGCTACGGATGAA-3.