This document is a user guide for the Ion AmpliSeq™ Library Kit 2.0, which can be used with the Ion AmpliSeq™ Direct FFPE DNA Kit, Ion Torrent™ Dual Barcode Kit 1-96, IonCode™ Barcode Adapters 1-384 Kit, Ion Xpress™ Barcode Adapters Kits, Ion Library Equalizer™ Kit, Ion AmpliSeq™ Ready-to-Use Panels, Ion AmpliSeq™ Made-to-Order and Community Panels, Ion AmpliSeq™ On-Demand Panels, and Ion AmpliSeq™ Sample ID Panel.