The user guide provides protocols, workflow, safety, and troubleshooting for Thermo Fisher Scientific's PrepSEQ sample preparation kits.
This guide describes the PrepSEQ sample preparation kits, which use magnetic‑particle technology to extract DNA/RNA from Mycoplasma, MMV, or Vesivirus samples. It includes product descriptions, workflow steps, and how to select the appropriate protocol for your sample type.
The manual covers detailed protocols (1‑2‑3, large‑scale, 3‑in‑1), safety and handling guidelines for magnetic particles, troubleshooting tips, required materials, and warranty information.
| Sample volume for 1‑2‑3 protocol | 100 µL (up to 10⁶ cells) |
|---|---|
| Sample volume for large‑scale protocol | up to 50 mL (up to 2×10⁸ cells) |
| Magnetic particle incubation | 37 °C for at least 10 minutes |
The 1‑2‑3 protocol processes 100 µL of sample, which can contain up to 10⁶ cells.
Magnetic particles should be incubated at 37 °C for at least 10 minutes with intermittent vortexing, and the pellet must be kept oriented toward the magnet in the magnetic stand.