One-step dual CRISPR/Cas9 guide RNA cloning protocol

Update: 16 November, 2023

We describe a novel CRISPR/Cas9 dual guide RNA cloning protocol. This protocol is rapid, inexpensive and allows re-usage of gRNA oligonucleotides. The protocol is applicable to cloning gRNAs into virtually any CRISPR/Cas9 backbone that allows cloning by Golden Gate.

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MD5 Checksum: 78E2FDF7E4DD3876BDB30997A2133BBE

Publication date: 16 November, 2023

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PDF Link: One-step dual CRISPR/Cas9 guide RNA cloning protocol PDF