Update: 30 September, 2023
This paper introduces a new multidimensional separation technique to reduce the complexity of complex serum samples. The technique uses a high-capacity multiple affinity removal column to specifically remove six high-abundance proteins (albumin, IgG, transferrin, haptoglobin, IgA, and anti-trypsin), and then uses a macro-porous reverse-phase C18 (mRP-C18) column to fractionate the depleted serum. Under the optimized mRP-HPLC condition, protein recoveries for depleted serum samples were greater than 99%. Reproducibility among patient samples was excellent based on the UV trace overlays. The mRP-C18 fractionated serum samples were further analyzed by 1D-PAGE and 2D-LC-MS/MS, the results showed that the technique can successfully reduce the complexity of serum samples and improve the detection sensitivity of low-abundance proteins.
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MD5 Checksum: 93903AA1C6A06BAEBCD2C8225E90FDB9
Publication date: 02 July, 2012
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