Key facts
- Manuals
- 1
- Languages
- JA
- Last added
- 2012-01-12
Featured SmaI内切酶 manuals
SmaI User Manual
The document describes the SmaI restriction endonuclease, including its recommended reaction conditions, buffer formulations, and handling procedures. It lists the product codes (e.g., D6633) and provides detailed instructions for setting up digestion reactions, enzyme activity units, and incubation times.
Safety and storage recommendations are also covered, advising storage at -20 °C and handling with disposable gloves. The manual includes buffer recipes such as 10 mM Tris‑HCl (pH 7.4), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA, 50 % glycerol, and a 10 X Buffer Y formulation. This manual is written in Japanese.
Covers: Product Overview · Reaction Conditions · Buffer Preparation · Storage and Stability · Safety Precautions · Digestion Protocol
Latest SmaI内切酶 manuals
SmaI User Manual
User Manual 1 pages · 123 KB · JAThis manual provides usage instructions, buffer recipes, storage conditions, and activity guidelines for the SmaI restriction enzyme.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in SmaI内切酶 manuals
What temperature should I use for optimal SmaI activity?
The enzyme shows 100 % activity at 23 °C; activity drops at higher temperatures.
From: SmaI User Manual
What is the composition of the 10 X Buffer Y for SmaI reactions?
The 10 X Buffer Y contains 33 mM Tris‑acetate (pH 7.9 at 37 °C), 10 mM magnesium acetate, 66 mM potassium acetate, and 0.1 mg/ml BSA.
From: SmaI User Manual