Key facts
- Manuals
- 1
- Languages
- ZH
- Last added
- 2012-01-12
Featured SacI内切酶 manuals
SacI 500U Endonuclease Manual
SacI is an imported restriction endonuclease supplied in 500U units. The product sheet lists the packaging, recognized DNA sequence (GAGCT^C / C^TCGAG), and basic buffer compatibility. Storage solution and buffer compositions are given in detail, and the activity unit is defined.
The manual explains how to store the enzyme at -20 °C, safety precautions for handling, and provides step‑by‑step protocols for single‑enzyme digests as well as double or multiple‑enzyme digests, including recommended reaction volumes, incubation temperatures, and buffer usage. This manual is written in Chinese.
Covers: Product Overview · Packaging · Storage Solution · Buffer Composition · Enzyme Activity Definition · Safety Precautions
Latest SacI内切酶 manuals
SacI 500U Endonuclease Manual
User Manual 1 pages · 120 KB · ZHThe SacI 500U endonuclease manual provides product details, storage, buffer recipes, and step‑by‑step usage instructions.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in SacI内切酶 manuals
What DNA sequence does SacI recognize?
SacI recognizes the sequence GAGCT^C (or its complement C^TCGAG).
Which buffer is recommended for SacI digests?
Use 1X Buffer SacI, which contains 10 mM Bis‑Tris Propane‑HCl (pH 6.5 at 37 °C), 10 mM MgCl₂, and 0.1 mg/ml BSA.