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- 2011-12-16
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Takara DNase I (RNase Free) User Manual
The Takara DNase I (RNase Free) enzyme is a highly purified DNase that lacks RNase activity, making it suitable for applications where RNA integrity must be preserved. It is supplied as a lyophilized powder (1,000 U) together with a 10× DNase I buffer.
The enzyme should be stored at –20 °C. The 10× buffer contains Tris‑HCl (20 mM, pH 7.5), NaCl (50 mM), CaCl₂ (0.1 mM) and 50 % glycerol. Typical protocols describe mixing the enzyme with the buffer and incubating at 37 °C for 20–30 min, followed by inactivation with EDTA and heat treatment. This manual is written in Chinese.
Covers: Package Contents · Storage Conditions · Buffer Composition · Enzyme Activity · Usage Protocol · Inactivation Procedure
Latest DNase manuals
Takara DNase I (RNase Free) User Manual
User Manual 2 pages · 176 KB · ZHTakara DNase I (RNase Free) manual provides enzyme description, buffer composition, usage instructions, and inactivation methods.
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User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in DNase manuals
What is the composition of the 10× DNase I buffer?
The buffer contains Tris‑HCl 20 mM (pH 7.5), NaCl 50 mM, CaCl₂ 0.1 mM and 50 % glycerol.
How can the DNase I reaction be stopped?
Add 2.5 mM EDTA and heat the mixture at 80 °C for 2 minutes to inactivate the enzyme.