Key facts
- Manuals
- 1
- Languages
- ZH
- Last added
- 2012-01-04
Featured Convenient manuals
Toyobo Target Clone™ -Plus- (TAK-101/TAK-201) User Manual
This manual describes the TA cloning system developed by Toyobo for rapid and reliable insertion of PCR products into the pTA2 vector. It covers the A‑attachment Mix reaction, ligation setup, transformation of competent cells, and verification of recombinant colonies.
Detailed protocols are given for PCR product preparation, optional A‑addition, ligation conditions, competent‑cell handling, heat‑shock transformation, and colony PCR screening. Safety notes and storage recommendations for reagents are also included. This manual is written in Chinese.
Covers: Introduction · Operation Instructions · A‑attachment Mix Features · Ligation Procedure · Transformation Procedure · Recombinant Confirmation
Latest Convenient manuals
Toyobo Target Clone™ -Plus- (TAK-101/TAK-201) User Manual
User Manual 17 pages · 684 KB · ZHThe Toyobo Target Clone™ -Plus- manual provides step‑by‑step instructions for TA cloning of PCR products, including ligation, transformation and colony…
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in Convenient manuals
How long does the ligation reaction take?
The ligation reaction can be completed in as little as 5 minutes.
From: Toyobo Target Clone™ -Plus- (TAK-101/TAK-201) User Manual
What is the maximum insert size that can be cloned?
Up to 12 kb PCR products can be cloned using this system.
From: Toyobo Target Clone™ -Plus- (TAK-101/TAK-201) User Manual