Vosshall Laboratory Manuals

Vosshall Laboratory Aedes aegypti CRISPR‑Cas9 Genome‑Engineering Protocol

This protocol guide describes the complete workflow for generating precise genomic alterations in Aedes aegypti using the CRISPR‑Cas9 system. It covers target design, sgRNA synthesis, Cas9 source selection, donor DNA preparation, injection mix cleanup, microinjection, and downstream screening.

The document also lists recommended reagents, detailed reaction conditions, and troubleshooting tips, enabling researchers to reproduce the method in their own laboratories.

Covers: Goals · General considerations · Selected Kits & Reagents · Target design and selection · sgRNA template generation and RNA production · In vitro transcription of sgRNAs