Proassay Manuals

Proassay PAE-005 Prescission Protease Material Data Sheet

Prescission Protease is a fusion protein composed of human rhinovirus 14 3C protease and GST, engineered to specifically recognize and cleave the short peptide Leu‑Glu‑Val‑Leu‑Phe‑Gln/Gly‑Pro at the Gln and Gly residues. The enzyme is produced by recombinant expression in Escherichia coli.

The sheet defines one activity unit as the amount of enzyme that, at 5 °C for 16 h, cleaves at least 90 % of 100 µg GST‑tagged fusion protein. Recommended reaction conditions include a 4 °C incubation for 15–16 h using a 10X Cleavage Buffer (Tris‑HCl, NaCl, EDTA, DTT, glycerin). For storage, keep the enzyme long‑term at –80 °C for up to 2 years or short‑term at –20 °C for up to 6 months, avoiding repeated freeze‑thaw cycles. This manual is written in Chinese.

Covers: Product Overview · Enzyme Activity Definition · Solution Composition · Recommended Reaction System · Storage Conditions · Reaction Component Composition