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- Languages
- EN
- Last added
- 2026-09-28
Featured 4A Biotech manuals
4A Biotech SPP102-100U/500U Goat anti-Rabbit IgG (H+L) PE Conjugated Product Data Sheet
The PE‑conjugated goat anti‑Rabbit IgG (H+L) antibody from 4A Biotech is an affinity‑purified reagent used for flow cytometry and other immunoassays. It is supplied in two sizes, 100 µg (catalog SPP102-100U) and 500 µg (catalog SPP102-500U), and is labeled with phycoerythrin for fluorescence detection.
The product should be stored at 2‑8 °C, protected from light, and never frozen. It is supplied in a phosphate‑buffered solution (pH 7.4) containing 0.09 % sodium azide, with a concentration of 1 mg ml⁻¹. Safety warnings note the presence of toxic NaN₃, requiring protective equipment during handling.
Covers: Storage Condition · Concentration · Storage Buffer · Applications · Experimental Methods · Catalog Numbers
4A Biotech Cell Counting Kit -8 (CCK-8) FXP132 Technical Data Sheet
The Cell Counting Kit‑8 (CCK‑8) is a water‑soluble tetrazolium salt that is reduced by mitochondrial dehydrogenases in viable cells to a yellow formazan dye. The intensity of the color is proportional to the number of living cells, allowing quantification of cell viability and proliferation.
The sheet provides detailed instructions for preparing standard curves, performing cell viability and toxicity assays in 96‑well plates, storage guidelines (0‑5 °C for one year, –20 °C for two years), and a comprehensive FAQ that addresses common experimental issues such as reference wavelength settings, blank controls, and interference from reagents.
Covers: Product Overview · Applications · Storage Conditions · Usage Method · Cell Viability Assay · Cell Proliferation and Toxicity Assay
Latest 4A Biotech manuals
4A Biotech SPP102-100U/500U Goat anti-Rabbit IgG (H+L) PE Conjugated Product Data Sheet
Product Leaflet 2 pages · 190 KB · ENTechnical data sheet for 4A Biotech's PE‑conjugated goat anti‑Rabbit IgG (H+L) antibodies, covering storage, concentration, applications and safety.
4A Biotech Cell Counting Kit -8 (CCK-8) FXP132 Technical Data Sheet
Datasheet 7 pages · 330 KB · ENThe 4A Biotech Cell Counting Kit‑8 (FXP132) technical data sheet describes its principle, usage, storage conditions and FAQs for cell proliferation and…
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Questions answered in 4A Biotech manuals
What temperature should the antibody be stored at?
Store the antibody at 2‑8 °C and do not freeze it; it is light‑sensitive.
From: 4A Biotech SPP102-100U/500U Goat anti-Rabbit IgG (H+L) PE Conjugated Product Data Sheet
What is the concentration of the antibody solution?
The solution concentration is 1 mg per milliliter.
From: 4A Biotech SPP102-100U/500U Goat anti-Rabbit IgG (H+L) PE Conjugated Product Data Sheet
Do I need to set a reference wavelength for the CCK‑8 assay?
It is not required; the CCK‑8 reagent has no absorbance at the reference wavelength, but setting one can help remove background from turbid samples.
From: 4A Biotech Cell Counting Kit -8 (CCK-8) FXP132 Technical Data Sheet
How should I prepare a blank control for the assay?
Use wells that contain culture medium and CCK‑8 but no cells; if a drug is present, include the drug in the blank as well.
From: 4A Biotech Cell Counting Kit -8 (CCK-8) FXP132 Technical Data Sheet