New England BioLabs Manuals

New England Biolabs OneTaq Hot Start DNA Polymerase M0481S Datasheet

OneTaq Hot Start DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases with an aptamer‑based inhibitor that prevents activity below 45 °C. The enzyme is supplied at 200 units (5,000 U ml⁻¹) and can be stored at –20 °C. It includes two 5× reaction buffers (Standard and GC) and a High GC Enhancer to improve yields for GC‑rich templates.

The datasheet provides detailed buffer compositions, unit definition, recommended enzyme concentrations, Mg²⁺ requirements, and thermocycling guidelines. It also lists applications such as high‑sensitivity, high‑throughput, GC‑rich, AT‑rich, colony and long PCR (up to ~6 kb). Companion products and licensing information are included.

Covers: Certificate of Analysis · Description · Applications · Reagents Supplied with Enzyme · Reaction Conditions · Unit Definition