Protocol Prediction and Validation of Native and Engineered Cas9 Guide Sequences

Update: 29 November, 2023

This article describes a protocol for predicting and validating native and engineered Cas9 guide sequences. The protocol includes the following steps: (1) Identify the PAM sequence of the target gene; (2) Design the guide RNA sequence; (3) Synthesize the guide RNA; (4) Transfect the target cells; (5) Assess the editing efficiency.

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Size: 386 KB

MD5 Checksum: A8C6D8702BEDB63501C79E43C44F3C2B

Publication date: 29 November, 2023

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